The widespread use of artificial intelligence (AI) has been growing in various fields. AI is defined as human-like automation in place of human beings that can operate many functions based on some level of intelligence. In education, AI offers powerful pedagogical tools that can help enhance instructional quality. Given the inevitable advancements of AI in education, this study aims to investigate teachers’ AI anxiety levels based on various demographic factors. For this purpose, the AI Anxiety Scale is adapted into Turkish, which provides a good fit of the model to the data for the construct validity. Moreover, the reliability coefficients of the scale show strong evidence of consistency in teachers’ responses to the items. For sociotechnical blindness dimension, male and female teachers do not show any significant differences. However, for learning, job replacement, AI configuration dimensions and the total scale, female teachers are more anxious towards AI than male teachers. Moreover, there is no difference observed based on degree levels teachers hold. Additionally, anxiety levels of teachers are not related to teachers’ age and years of experience in teaching.